Isolation of Shigellae

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Isolation of Shigellae

The efficiencies of three enrichment broths and four plating media for isolation of enteric pathogens were compared from 1,117 stool specimens. Direct streaking proved to be inferior to enrichment, detecting only 50% of the salmonellae and 61% of the shigellae. By contrast, Selenite Broth (SF) found 90% of the total salmonellae isolates and 82% of the shigellae isolates. Gram-Negative Broth (GN...

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Isolation of shigellae. V. Comparison of enrichment broths with stools.

Many enteric media are more efficient for the detection of salmonellae than of shigellae. Comparisons of three enrichment broths and three plating media were made during analysis of 1,405 stool specimens to choose a combination of media which would enhance detection of shigellae as well. Gram-Negative (GN), Selenite, and Silliker's Broths were streaked to E M B, Salmonella-Shigella (SS), and xy...

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Isolation of Salmonellae and Shigellae from an artificial mixture of fecal bacteria.

Numerous selective media, available commercially, act by suppressing "normal" bacterial inhabitants of the intestine while permitting the growth of so-called pathogenic representatives of the family Enterobacteriaceae. This investigation attempts to evaluate the action of Salmonella-Shigella (SS) agar, xylose lysine desoxycholate (XLD) agar, and hektoen enteric (HE) agar. Salmonellae and shigel...

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Purine requiring strains of Shigellae.

The importance of growth factors in the nutrition of shigellae was recognized by Koser and Saunders (1935) and it was shown that the growth factors present in tissue extract could be replaced by nicotinic acid (Koser et al., 1938). The importance of nicotinic acid and nicotinamide as growth factors in the nutrition of shigellae has been shown by Dorfman et al. (1939). Reports of other vitamins ...

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Growth of shigellae in monolayer tissue cultures.

Gerber, Dolores F. (University of California, Berkeley) and H. M. S. Watkins. Growth of shigellae in monolayer tissue cultures. J. Bacteriol. 82:815-822. 1961.-The influence of environment on virulence, antigenicity, and antibiotic susceptibility of infectious agents is well recognized. The development of tissue culture monolayer techniques has stimulated new interest in manifestations of these...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1971

ISSN: 0003-6919

DOI: 10.1128/am.21.1.32-37.1971